Dr. Hossam Taha, lecturer at the Faculty of Biotechnology, organized an enlightening session for all assistan staff on the sheer principles of Real-Time PCR. His session encompassed the elaborations on essential applications for utilizing this technique.
First and foremost, he emphasized how real time pcr is useful for relative gene expression both quantitatively, in estimating the number of copies of the gene of interest in both mutant and normal samples, and qualitatively, in detecting the presence or absence of certain genes. Moreover, the session also entitled the main principles in undergoing efficiently mini-prep extraction procedures for extracting intact DNA and RNA samples.
Last but not least, valuable information on understanding the concepts of data analysis retrieved from real time pcr trials were also discussed. In the end, all the assisting staff were overwhelmed by such an event, where they were utterly enthusiastic for another practical session on undergoing a comparative trial on genes of interest and analyzing their results in forthcoming encounters.